anti vimentin Search Results


vimentin  (Developmental Studies Hybridoma Bank)
95
Developmental Studies Hybridoma Bank vimentin
Vimentin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vimentin/product/Developmental Studies Hybridoma Bank
Average 95 stars, based on 1 article reviews
vimentin - by Bioz Stars, 2026-02
95/100 stars
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antibody production recombinant human vimentin rhvim  (R&D Systems)
93
R&D Systems antibody production recombinant human vimentin rhvim
Antibody Production Recombinant Human Vimentin Rhvim, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody production recombinant human vimentin rhvim/product/R&D Systems
Average 93 stars, based on 1 article reviews
antibody production recombinant human vimentin rhvim - by Bioz Stars, 2026-02
93/100 stars
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vimentin  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology vimentin
Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization <t>of</t> <t>E-cadherin</t> (green) and <t>vimentin</t> (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.
Vimentin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vimentin/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
vimentin - by Bioz Stars, 2026-02
96/100 stars
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anti phospho vimentin ser56  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc anti phospho vimentin ser56
Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization <t>of</t> <t>E-cadherin</t> (green) and <t>vimentin</t> (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.
Anti Phospho Vimentin Ser56, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phospho vimentin ser56/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
anti phospho vimentin ser56 - by Bioz Stars, 2026-02
94/100 stars
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monoclonal mouse antibody  (Valiant Co Ltd)
90
Valiant Co Ltd monoclonal mouse antibody
Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization <t>of</t> <t>E-cadherin</t> (green) and <t>vimentin</t> (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.
Monoclonal Mouse Antibody, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monoclonal mouse antibody/product/Valiant Co Ltd
Average 90 stars, based on 1 article reviews
monoclonal mouse antibody - by Bioz Stars, 2026-02
90/100 stars
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vimentin  (Proteintech)
96
Proteintech vimentin
Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization <t>of</t> <t>E-cadherin</t> (green) and <t>vimentin</t> (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.
Vimentin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vimentin/product/Proteintech
Average 96 stars, based on 1 article reviews
vimentin - by Bioz Stars, 2026-02
96/100 stars
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antitwist  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc antitwist
Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization <t>of</t> <t>E-cadherin</t> (green) and <t>vimentin</t> (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.
Antitwist, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antitwist/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
antitwist - by Bioz Stars, 2026-02
96/100 stars
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vimentin  (Bioss)
94
Bioss vimentin
Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization <t>of</t> <t>E-cadherin</t> (green) and <t>vimentin</t> (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.
Vimentin, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vimentin/product/Bioss
Average 94 stars, based on 1 article reviews
vimentin - by Bioz Stars, 2026-02
94/100 stars
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vimentin  (Sino Biological)
94
Sino Biological vimentin
Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization <t>of</t> <t>E-cadherin</t> (green) and <t>vimentin</t> (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.
Vimentin, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vimentin/product/Sino Biological
Average 94 stars, based on 1 article reviews
vimentin - by Bioz Stars, 2026-02
94/100 stars
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vimentin  (R&D Systems)
93
R&D Systems vimentin
Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization <t>of</t> <t>E-cadherin</t> (green) and <t>vimentin</t> (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.
Vimentin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vimentin/product/R&D Systems
Average 93 stars, based on 1 article reviews
vimentin - by Bioz Stars, 2026-02
93/100 stars
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standard biotools 3143027d  (fluidigm)
95
fluidigm standard biotools 3143027d
Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization <t>of</t> <t>E-cadherin</t> (green) and <t>vimentin</t> (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.
Standard Biotools 3143027d, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/standard biotools 3143027d/product/fluidigm
Average 95 stars, based on 1 article reviews
standard biotools 3143027d - by Bioz Stars, 2026-02
95/100 stars
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antibodies mouse anti human monoclonal antibody  (Boster Bio)
96
Boster Bio antibodies mouse anti human monoclonal antibody
Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization <t>of</t> <t>E-cadherin</t> (green) and <t>vimentin</t> (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.
Antibodies Mouse Anti Human Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies mouse anti human monoclonal antibody/product/Boster Bio
Average 96 stars, based on 1 article reviews
antibodies mouse anti human monoclonal antibody - by Bioz Stars, 2026-02
96/100 stars
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Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization of E-cadherin (green) and vimentin (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.

Journal: Cancer cell

Article Title: Loss of p53 in enterocytes generates an inflammatory microenvironment enabling invasion and lymph node metastasis of carcinogen-induced colorectal tumors.

doi: 10.1016/j.ccr.2012.11.014

Figure Lengend Snippet: Figure 4. Activation of NF-kB Is Associated with Cxcl1 and Twist Upregulation in p53-Deficient Invasive Carcinoma (A) GSEA comparing all differentially regulated genes from AOM-induced invasive cancers in Tp53DIEC mice with a data set of NF-kB target genes obtained from microdissected invading human tumor epithelia (Horst et al., 2009). Normalized enrichment score: 1.46; p < 0.0001. (B) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (C–F) Immunohistochemical analysis of activated p-p65 (C), CXCL1 (D), F4/80 (E), and Gr-1 (F) in invasive cancers of AOM-challenged Tp53DIEC mice. Dashed white lines mark the basal membrane. Scale bars represent 50 mm. (G) Immunoblot analysis for the indicated proteins in lysates from invasive cancers from Tp53DIEC mice and noninvasive adenomas from Tp53F/F mice 16 weeks after the first AOM administration. (H) Immunohistochemical analysis of Twist in invasive cancers of AOM-challenged Tp53DIEC mice. The scale bar represents 50 mm. (I) Colocalization of E-cadherin (green) and vimentin (red) in invasive cancer epithelial cells of AOM-challenged Tp53DIEC mice, indicating EMT; nuclei are stained using 40,6-diamidino-2-phenylindole (blue). The scale bar represents 200 mm. (J–M) Representative immunohistochemical analysis of p53 (J and L) and p-p65 (K and M) in human colon cancer samples. Scale bars represent 100 mm. (N) Correlation of p53 staining and p-p65 staining (n = 59; c2 test, p < 0.05). (O) Correlation of p-p65 staining and the presence of lymph node (LN) metastases (n = 59; c2 test, p < 0.02). (P) Relative CD68 mRNA level in human colon cancer patients who were characterized by expression of both p53 and p-p65 (p53+/p-p65+; n = 23) or not (other; n = 30). Data are mean ± SE; *p < 0.05 by t test. (Q and R) Representative immunohistochemical staining of CD68 in human colon cancer patients who were characterized by expression of both p53 and phospho-p65 (Q) or the absence of p53 and phospho-p65 (R). Scale bars represent 100 mm. See also Figure S3.

Article Snippet: Standard immunohistochemical procedures were performed using the following antibodies: b-catenin (Santa Cruz; sc-1496), bromodeoxyuridine (BrdU) (AbD Serotec; MCA 2060), cleaved caspase-3 (Cell Signaling; 9661), CXCL1 (R&DSystems;MAB453), F4/80 (Caltag; MF48000), Gr-1 (eBioscience; 12-5931-82), phospho-Histone H2A.X (Cell Signaling; 2577), E-cadherin (Becton Dickinson; 610182), Twist (Abcam; 49254), vimentin (Santa Cruz; SC-7557), VE-cadherin (Santa Cruz; SC-6458), and occludin (Invitrogen; 711500).

Techniques: Activation Assay, Western Blot, Immunohistochemical staining, Membrane, Staining, Expressing